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Next Generation Sequencing (NGS)

This intermediate-level course provides a thorough overview of the field of next-generation sequencing, from its properties and strength and its major applications, to an overview of a typical sequencing workflow.

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About This Course

This intermediate level course on Next Generation Sequencing will provide you with a deep understanding of this technology and its varied applications. Students will be introduced to the development of this novel technique as well as learn about the details of a typical workflow, the different methods and applications of NGS with their associated advantages, and the common steps involved in data management and analysis. In addition, students will explore the evolution and growth of bioinformatics tools in order to get a grasp of the changing landscape of NGS and what that means for the future of genomics.

Who Is This Course For

This intermediate course is aimed at an audience interested in learning more about the NGS technology and its applications. While the course does contain an introduction to DNA’s structure and function alongside some of the basic genetic concepts, some previous knowledge in genetics and genomics may be useful to understand the in-depth technological advancements and analysis options that this tool both uses and provides. Overall, the content of this certification encompasses a broad range of topics linked to NGS and explores the current and emerging sequencing technologies alongside their advantages. A step-by-step approach of the NGS workflow and an overview of its varied applications will provide a solid overview to anyone interested in this technology.

What you'll learn

  • Review of the genetic concepts required to understand NGS and explore the development of the first generations of DNA sequencing methods
  • Explore and understand the motivation and fundamental basis of NGS, the current and emerging sequencing technologies alongside their advantages and potential biases that may affect their accuracy
  • Learn the typical workflow of NGS from sample preparation, to making high-quality libraries, to post-sequencing quality control and data management
  • Gain familiarity with the major application of NGS which include, among others, transcriptomic analysis by RNA-Seq, genomic variation discovery by Whole Genome Sequencing, and epigenomics analysis.
  • Discover what the future of NGS may hold by covering the current changing landscape of NGS and the rapid evolution of data analysis bioinformatics tools

Meet Your Instructor

Dr. Eléonore Fox

Eléonore is a PhD graduate in Medicine from the University of Cambridge. Her doctoral research focussed on respiratory diseases and intensive care. She has recently conducted a clinical research project on COVID-19 in order to analyse how the virus affects various types of immune cells in human blood. Prior to her completing her PhD, Eléonore has worked in cancer research for 4 years, both at the Dana Farber Cancer Institute of Harvard University and at the Rosalind and Goodman Research Center at McGill University. She also has experience of working in a pharmacological company, having worked for the Global Research and Development Department of Pfizer.

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