Creating Knock-out and Knock-in Using CRISPR/Cas9
This advanced course is designed for researchers planning gene knock-out and knock-in experiments using CRISPR/Cas9 across different cell lines. Divided into two segments—‘Creating Knock-out in E. coli’ and ‘Creating Knock-in Cell Lines’—it covers essential CRISPR/Cas9 concepts and prepares learners with the techniques and tools needed for successful lab execution.
£250.00
Starts 9th September 2026
Creating Knock-out and Knock-in Using CRISPR/Cas9
This advanced course is designed for researchers planning gene knock-out and knock-in experiments using CRISPR/Cas9 across different cell lines. Divided into two segments—‘Creating Knock-out in E. coli’ and ‘Creating Knock-in Cell Lines’—it covers essential CRISPR/Cas9 concepts and prepares learners with the techniques and tools needed for successful lab execution.
£250.00
Starts 9th September 2026
About This Course
- 8 Modules
- 6 Hours
- Level: Advanced
- Language: English
- Course Type: Self-paced
- CPD Accredited Certificate
- Online Learning
Who Is This Course For
This advanced-level, self-paced course is suitable for a wide range of learners, including undergraduate and postgraduate students, researchers, academics, and industry professionals with a background in life sciences. It is also designed for professionals looking to enhance their laboratory skills or become familiar with CRISPR/Cas9 technology. For industry professionals, the course can assist in career advancement and help secure desirable positions. A background in life science is essential, and prior knowledge of CRISPR/Cas9 genome editing is desirable but not required. Experience with plasmid construction and transfection is also helpful but not mandatory. Those who have completed the ‘CRISPR: Revolutionising Genome Editing’ course offered by CBEHx will find this advanced course particularly beneficial.
What you'll learn
- Learn about the basic concepts of CRISPR/Cas9 technology.
- Gain insight into the applications of CRISPR/Cas9 knock-out and knock-in cell lines.
- Learn how to construct plasmids, donors, and guide vectors.
- Learn how to transfect cells via electroporation.
- Gain insight into mutagenesis and plasmid curing processes.
- Learn about analytical methods, such as genotyping and direct sequencing, used to validate gene edits.
Course Content
Creating Knock-Out in E. coli Using CRISPR/Cas9
Meet Your Instructor
Dr. Veronica Hughes
Veronica Hughes received her Ph.D. in medical science from the University of Florida. Her primary interest was cancer metastasis, dysregulation of the tumour microenvironment, and analysis of clinical trial design. Additionally, she engaged in the development of novel small animal models.
Our Partners
We are proud to partner with the leading In2scienceUK as a step towards achieving our mission of imparting quality education and knowledge across the globe.
How This Course Supports Your Career Development
Biotechnology is one of the fastest-evolving industries in the world, creating demand for professionals with knowledge of emerging fields such as genomics, gene therapy, bioinformatics, artificial intelligence and precision medicine.
Our learners include students, graduates, researchers and professionals from leading universities, pharmaceutical companies, biotechnology organisations and research institutions across the UK and internationally.
Upon successful completion, learners receive a CPD-accredited certificate and earn recognised CPD hours that can be included on CVs, LinkedIn profiles, professional portfolios and CPD records.
By completing this course, you will strengthen your scientific knowledge, earn valuable CPD hours and demonstrate a commitment to continuous professional development within the life sciences sector.
INTERESTED IN THIS COURSE FOR YOUR ORGANISATION?
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Usually, there are no general prerequisites when enrolling for any individual courses on cbehx.co.uk. However, some courses are certainly more advanced than others that are of introductory and intermediate level. For advanced-level courses, prior knowledge on the topic will be helpful for understanding complex concepts but not mandatory.
The relevant information about the suggested knowledge and background that might be helpful to follow each course is provided on the respective course registration page.
Yes, the courses are flexible and self-paced to suit your schedule. The term “self-paced” implies that the courses do not follow a pre-assigned schedule for learning within the course duration. You will be able to access all the course materials for each course that you have enrolled in as soon as the course begins.
The course completion certificate is CPD accredited. For more informations please visit https://cpduk.co.uk
