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Creating Knock-out and Knock-in Using CRISPR/Cas9

This advanced-level course is precisely tailored to assist researchers planning to conduct gene knock-out and knock-in experiments in different cell lines using the ground-breaking CRISPR/Cas9 technology. This course comprises two segments- ‘Creating Knock-out in E. coli Using CRISPR/Cas9’ and ‘Creating Knock-in Cell Lines Using CRISPR/Cas9’. Overall, the course will teach researchers the basic concepts of CRISPR/Cas9 technology and help them get familiarised with the laboratory-based techniques and instruments required before they actually conduct the experiment.


Starts 29th May 2024

About This Course

CRISPR/Cas9 is a path-breaking genome editing technology that helps in achieving precise and targeted genome editing in eukaryotic and prokaryotic cell lines. It offers high precision, efficiency, and simplicity in experimental design. This versatile genome editing technology was first discovered in prokaryotes and archaea and later used in eukaryotic systems to achieve precise gene edits. CRISPR/Cas9 has become one of the most popularly used genome editing technologies. However, to be able to conduct a CRISPR/Cas9 mediated gene knock-out or knock-in experiment efficiently in the laboratory, a researcher first needs to understand the basic concepts of CRISPR/Cas9 technology and its components. This course provides an overview of the CRISPR/Cas9 technology and discusses the protocol for creating gene knock-out and gene knock-in followed by the validation of CRISPR/Cas9 knock-out and knock-in. The course also provides insight into the applications of CRISPR knock-out and knock-in and shares important tips that will assist the researcher in conducting the experiment successfully in the laboratory. This course also provides useful tips and discusses the common causes that can lead to unsuccessful experiments. Each module of the course is equipped with a quiz section that will help to evaluate the level of learning and boost the confidence of those undertaking the course.

Who Is This Course For

This self-paced advanced-level course is designed for a wide array of learners ranging from undergraduate and postgraduate students, researchers, and academicians, to industry professionals with a background in life sciences. This course is also for professionals looking to brush up on their laboratory skills or familiarise themselves with the CRISPR/Cas9 technology. For industry professionals, this course will assist in career advancements and landing desired jobs. A background in life science is essential and basic knowledge of CRISPR/Cas9 genome editing technology is desirable. Additionally, experimental knowledge of plasmid construction and transfection is desirable but not mandatory. Those who have attended the ‘CRISPR: Revolutionising Genome Editing’ course offered by CBEHx will benefit from the advanced-level information presented in this course.

What you'll learn

  • Learn about the basic concepts of the CRISPR/Cas9 technology
  • Gain insight into the applications of CRISPR/Cas9 knock-out and knock-in cell lines
  • Learn how to construct plasmids, donors, and guide vectors
  • Learn how to transfect cells via electroporation
  • Get an insight into mutagenesis and plasmid curing processes
  • Get an overview of analytical methods such as genotyping and direct sequencing that are used for validating the gene edit

Course Content

Expand All
Creating Knock-Out in E. coli Using CRISPR/Cas9
Creating Knock-in in cell Lines Using CRISPR/Cas9

Meet Your Instructor

Dr. Veronica Hughes

Veronica Hughes received her Ph.D. in medical science from the University of Florida. Her primary interest was cancer metastasis, dysregulation of the tumour microenvironment, and analysis of clinical trial design. Additionally, she engaged in the development of novel small animal models.

Our Partners

We are proud to partner with the leading In2scienceUK as a step towards achieving our mission of imparting quality education and knowledge across the globe.

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Usually, there are no general prerequisites when enrolling for any individual courses on cbehx.co.uk. However, some courses are certainly more advanced than others that are of introductory and intermediate level. For advanced-level courses, prior knowledge on the topic will be helpful for understanding complex concepts but not mandatory.

The relevant information about the suggested knowledge and background that might be helpful to follow each course is provided on the respective course registration page.

Yes, the courses are flexible and self-paced to suit your schedule. The term “self-paced” implies that the courses do not follow a pre-assigned schedule for learning within the course duration. You will be able to access all the course materials for each course that you have enrolled in as soon as the course begins.

The course completion certificate is CPD accredited. For more informations please visit https://cpduk.co.uk